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Universal HIS in the sample or standard competes with a fixed amount of Universal HIS on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal HIS
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP1g
only those wells that contain Human CCL16
The plate has been pre-coated with Mouse FAT3 antibody
Mouse EPO (Erythropoietin) ELISA Kit BSAP Universal HIS in the sampleThe Mouse (EPO) Erythropoietin ELISA Kit measures Erythropoietin in Mouse samples. The plate has been pre coated with Mouse EPO antibody. EPO present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse EPO Antibody is added and binds to EPO in the sample. Then Streptavidin HRP is added and binds to the Biotinylated EPO antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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