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And then biotinylated Canine ANG-2 Antibody is added and binds to ANG-2 in the sample
Substrate solution is then added and color develops in proportion to the amount of Human KALRN
The concentration of Mouse MIP1b in the samples is then determined by comparing the OD of the samples to the standard curve
GRP present in the sample is added and binds to antibodies coated on the wells
Human LAYN (Layilin) ELISA Kit Packaging:96 wells And then biotinylated Canine ANG-2The Human (LAYN) Layilin ELISA Kit measures Layilin in samples. The plate has been pre coated with Human LAYN antibody. LAYN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LAYN Antibody is added and binds to LAYN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LAYN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
