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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit IFNb
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TNFa
The concentration of Human PLGF in the samples is then determined by comparing the OD of the samples to the standard curve
NTN4 present in the sample is added and binds to antibodies coated on the wells
Mouse OPN (Osteopontin) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Mouse (OPN) Osteopontin ELISA Kit measures Osteopontin in Mouse samples. The plate has been pre coated with Mouse SPP1 antibody. SPP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SPP1 Antibody is added and binds to SPP1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SPP1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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