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The concentration of Mouse ACTa2 in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Human FGg in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Rat MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
RESISTIN present in the sample is added and binds to antibodies coated on the wells
Mouse GAL3 (Galectin 3) ELISA Kit Packaging:96 wells The concentration of Mouse ACTa2The Mouse (GAL3) Galectin 3 ELISA Kit measures Galectin 3 in Mouse samples. The plate has been pre coated with Mouse GAL3 antibody. GAL3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse GAL3 Antibody is added and binds to GAL3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GAL3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
