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only those wells that contain Human VCAM1 and HRP-conjugated antibody will exhibit a change in color
Then a biotinylated detection antibody specific for Human AQP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VEGF-A
Human ANP in the sample or standard competes with a fixed amount of Human ANP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human ANP
Human ADH (Antidiuretic Hormone) ELISA Kit NA1/NA2 only those wells that containThe Human ADH (Antidiuretic Hormone) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human ADH. During the reaction, Human ADH in the sample or standard competes with a fixed amount of Human ADH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human ADH. Excess conjugate and unbound sample or standard are washed away, and Avidin Horseradish
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