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Then a biotinylated detection antibody specific for Mouse FAS/CD95 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL1a
The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of FGF23 in the specimen
Then Streptavidin-HRP is added and binds to the Biotinylated COX6C antibody
Human LAMA3 (Laminin Subunit Alpha-3) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (LAMA3) Laminin Subunit Alpha 3 ELISA Kit measures Laminin Subunit Alpha 3 in samples. The plate has been pre coated with Human LAMA3 antibody. LAMA3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LAMA3 Antibody is added and binds to LAMA3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LAMA3 antibody. After incubation unbound Streptavidin HRP is washed away
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