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Then Streptavidin-HRP is added and binds to the Biotinylated NFASC antibody
Then a biotinylated detection antibody specific for Rat NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of IL-29 in the sample was calculated by drawing a standard curve
Calculate the concentration of HAMP in the sample by plotting standard curve
Rat CKMB (Creatine Kinase MB Isoenzyme) QuickTest ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Rat CKMB (Creatine Kinase MB Isoenzyme) QuickTest ELISA Kit measures CKMB in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples
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