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The concentration of Cattle FGF2 in the samples is then determined by comparing the OD of the samples to the standard curve
coli expression system and the target gene encoding Ala2-Asp135 is expressed with a 6His tag at the C-terminus
The OD value is proportional to the concentration of Human C5
Then a biotinylated detection antibody specific for Mouse IFN-Beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
VIPR1 (Vasoactive Intestinal Peptide Receptor 1) ELISA Kit (Small Sample) CCPBP The concentration of Cattle FGF2The VIPR1 (Vasoactive Intestinal Peptide Receptor 1) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human VIPR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human VIPR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB
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