Human IL-22 (Interleukin-22) ELISA Kit Packaging:96 wells 12-HETE in the sample or

12-HETE in the sample or standard competes with a fixed amount of biotin-labeled 12-HETE for sites on a pre-coated antibody specific to 12-HETE

Then a biotinylated detection antibody specific for Human TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

And then biotinylated Human CCL26 Antibody is added and binds to CCL26 in the sample

Then a biotinylated detection antibody specific for Mouse ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Human IL-22 (Interleukin-22) ELISA Kit Packaging:96 wells 12-HETE in the sample orThe Human IL 22 (Interleukin 22) ELISA Kit measures IL 22 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 22 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection