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The Rat PKBa (Protein Kinase B Alpha) ELISA Kit is a Sandwich enzyme immunoassay
Rat SST in the sample or standard competes with a fixed amount of Rat SST on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat SST
The concentration of Rat BMP2 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for IL-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human AD (Adipolin) ELISA Kit Packaging:96 wells The Rat PKBa (Protein KinaseThe Human (AD) Adipolin ELISA Kit measures Adipolin in samples. The plate has been pre coated with Human C1QTNF12 antibody. C1QTNF12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human C1QTNF12 Antibody is added and binds to C1QTNF12 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated C1QTNF12 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
