Human CDK4 (Cyclin Dependent Kinase 4) QuickTest ELISA Kit Packaging:96 wells coli expression system and the

coli expression system and the target gene encoding Ala2-Asp135 is expressed with a 6His tag at the C-terminus

You can calculate the concentration of Human DS in the samples by comparing the OD of the samples to the standard curve

Then Streptavidin-HRP is added and binds to the Biotinylated KCNK3 antibody

Substrate solution is then added and color develops in proportion to the amount of Human VKORC1

Human CDK4 (Cyclin Dependent Kinase 4) QuickTest ELISA Kit Packaging:96 wells coli expression system and theThe Human CDK4 (Cyclin Dependent Kinase 4) QuickTest ELISA Kit measures CDK4 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples