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Substrate solution is then added and color develops in proportion to the amount of Rat MMP2
LFA-1 present in the sample is added and binds to antibodies coated on the wells
Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of Cortisol in the sample
a capture antibody-ERRFI1-biotin-detection antibody complex was formed
Human GAL7 (Galectin-7) ELISA Kit pp1158 Substrate solution is then addedThe Human GAL7 (Galectin 7) ELISA Kit measures GAL7 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti GAL7 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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