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Then a biotinylated detection antibody specific for Mouse PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse IL-12p40
Human Ang-â…¡ in the sample or standard competes with a fixed amount of Human Ang-â…¡ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang-â…¡
and the color intensity is directly proportional to the amount of Human CHL1 present
Monomeric CRP ELISA SL-1 Then a biotinylated detection antibodyThe monomeric CRP (mCRP) ELISA is an enzyme immunoassay for the quantitative determination of monomeric CRP (mCRP) in human serum and plasma. Human Monomeric ELISA specifically determines monomeric CRP (mCRP) without any reactivity with pentameric CRP (pCRP). The microtiter plate is coated with the antibody specifically binding the mCRP. The human serum and plasma are incubated in the plate with the capture antibody. The specimen is washed out and the
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