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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PF4
Rat Beta-CTx in the sample or standard competes with a fixed amount of Rat Beta-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat Beta-CTx
eliminating the need for cloning
You can calculate the concentration of Rat KISS1 in the samples by comparing the OD of the samples to the standard curve
Human HIST1H1C (Histone H1.2) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (HIST1H1C) Histone H1. 2 ELISA Kit measures Histone H1. 2 in samples. The plate has been pre coated with Human HIST1H1C antibody. HIST1H1C present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HIST1H1C Antibody is added and binds to HIST1H1C in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HIST1H1C antibody. After incubation unbound Streptavidin HRP is washed away
