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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IPF
a horseradish peroxidase conjugated mouse anti-Human B4GALT1 Antibody is added to the wells and binds to B4GALT1 in the sample
The Rabbit IL-18 ELISA kit is a sandwich enzyme immunoassay
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 37 nm
Human ACO1 (Cytoplasmic Aconitate Hydratase) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (ACO1) Cytoplasmic Aconitate Hydratase ELISA Kit measures Cytoplasmic Aconitate Hydratase in samples. The plate has been pre coated with Human ACO1 antibody. ACO1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ACO1 Antibody is added and binds to ACO1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ACO1 antibody. After incubation unbound Streptavidin HRP is
