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CP present in the sample is added and binds to antibodies coated on the wells
The OD value is proportional to the concentration of Mouse CKMB
And then biotinylated Human TRAF6 Antibody is added and binds to TRAF6 in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EMR1
Human SPARC/ON (Osteonectin) ELISA Kit UGB CP present in the sampleThe Human SPARC ON (Osteonectin) ELISA Kit measures ON in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ON antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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