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You can calculate the concentration of VCAM-1 in the samples by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated NRCAM antibody
The concentration of Human MCP-1 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ELA3B
Rat LUM (Lumican) ELISA Kit Packaging:96 wells You can calculate the concentrationThe Rat (LUM) Lumican ELISA Kit measures Lumican in Rat samples. The plate has been pre coated with Rat LUM antibody. LUM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LUM Antibody is added and binds to LUM in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LUM antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution is
