Human CLDN4 (Claudin 4) ELISA Kit Hpt Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Rat NEUROG1

F7 present in the sample is added and binds to antibodies coated on the wells

Then a biotinylated detection antibody specific for Human F7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

only those wells that contain Mouse BMP3

Human CLDN4 (Claudin 4) ELISA Kit Hpt Substrate solution is then addedThe Human CLDN4 (Claudin 4) ELISA Kit measures CLDN4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CLDN4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.