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You can calculate the concentration of Rat sCD86 in the samples by comparing the OD of the samples to the standard curve
Only those wells that contain Mouse GRN
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SHH
Substrate solution is then added and color develops in proportion to the amount of Human PCK2
Human ASIP (Agouti-signaling protein) ELISA Kit Packaging:96 wells You can calculate the concentrationThe Human ASIP (Agouti signaling protein) ELISA Kit measures ASIP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ASIP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
