Porcine CPP (Copeptin) ELISA Kit alpha-intermedin Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Human KCNK4

and the Human VEGFA standard plate wells that pre-coated using protein-related techniques are provided separately

Then Streptavidin-HRP is added and binds to the Biotinylated PKP3 antibody

Then a biotinylated detection antibody specific for Human TIMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Porcine CPP (Copeptin) ELISA Kit alpha-intermedin Substrate solution is then addedThe Porcine CPP (Copeptin) ELISA Kit measures CPP in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CPP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.