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a horseradish peroxidase conjugated mouse anti-Human SPINK4 Antibody is added to the wells and binds to SPINK4 in the sample
Then a biotinylated detection antibody specific for Rat IL-1R1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
When the sample containing IL-6 is added to the plate
The OD value is proportional to the concentration of Mouse CTSK
Mouse CD14 (Cluster Of Differentiation 14) ELISA Kit (Small Sample) Packaging:48 wells a horseradish peroxidase conjugated mouseThe Mouse CD14 (Cluster Of Differentiation 14) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Mouse CD14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Mouse CD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
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