Equine FS (Follistatin) ELISA Kit Packaging:96 wells 100uL of TMB Reagent is

100uL of TMB Reagent is added and incubated at room temperature for 15 minutes

This ELISA kit is designed to detect Human STK4

Only those wells that contain Mouse IGFBP-2

Then a biotinylated detection antibody specific for Mouse MCP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Equine FS (Follistatin) ELISA Kit Packaging:96 wells 100uL of TMB Reagent isThe Equine (FS) Follistatin ELISA Kit measures Follistatin in Equine samples. The plate has been pre coated with Equine FST antibody. FST present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Equine FST Antibody is added and binds to FST in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FST antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.