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The plate has been pre-coated with Mouse TNNI1 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated SFRP2 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated U2 antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APOA5
Rat POMC (Pro-Opiomelanocortin) ELISA Kit Packaging:96 wells The plate has been pre-coatedThe Rat (POMC) Pro Opiomelanocortin ELISA Kit measures pro opiomelanocortin in Rat samples. The plate has been pre coated with Rat POMC antibody. POMC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat POMC Antibody is added and binds to POMC in the sample. Then Streptavidin HRP is added and binds to the Biotinylated POMC antibody. After incubation unbound Streptavidin HRP is washed away during a
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