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The concentration of Universal VD in tested samples can be calculated by comparing the OD of the samples to the standard curve
SERPINA1 present in the sample is added and binds to antibodies coated on the wells
and is thought to be transported there from the cerebrospinal fluid via endocytosis
And then biotinylated Human CADM1 Antibody is added and binds to CADM1 in the sample
Human C3 (Complement 3) ELISA Kit E2 The concentration of Universal VDThe Human (C3) Complement 3 ELISA Kit measures Complement 3 in samples. The plate has been pre coated with Human C3 antibody. C3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human C3 Antibody is added and binds to C3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated C3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
