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Calculate the concentration of PTF1A in the sample by plotting standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFa
CD9 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL6
Rat CALD (Caldesmon) ELISA Kit IFI10 Calculate the concentration of PTF1AThe Rat CALD (Caldesmon) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat CALD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CALD and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and
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