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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GDF10
Then Streptavidin-HRP is added and binds to the Biotinylated MOG antibody
Substrate solution is then added and color develops in proportion to the amount of Rat GPX2
And then biotinylated Rat MAPK7 Antibody is added and binds to MAPK7 in the sample
Rat S100A4 (S100 Calcium Binding Protein A4) ELISA Kit Packaging:96 wells Standards or samples are addedThe Rat (S100A4) S100 Calcium Binding Protein A4 ELISA Kit measures S100 Calcium Binding Protein A4 in Rat samples. The plate has been pre coated with Rat S100A4 antibody. S100A4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat S100A4 Antibody is added and binds to S100A4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated S100A4 antibody. After incubation unbound
