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The reaction is stopped by adding an acidic stop solution
The concentration of Rat VF in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Rat PTX-3/TSG-14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human CPLX2 in the samples is then determined by comparing the OD of the samples to the standard curve
TP73 (Tumor Protein p73) ELISA Kit (Small Sample) DC/B-CK The reaction is stopped byThe TP73 (Tumor Protein p73) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human TP73. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human TP73. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
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