Active Caspase 3 Mouse mAb Flt3-L Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Rat IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

only those wells that contain Human LTF and HRP-conjugated antibody will exhibit a change in color

Then a biotinylated detection antibody specific for Human IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Universal VMA in the sample or standard competes with a fixed amount of Universal VMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VMA

Active Caspase 3 Mouse mAb Flt3-L Then a biotinylated detection antibodyActivation of caspase 3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase 3 requires aspartic acid at the P1 position.