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Then Streptavidin-HRP is added and binds to the Biotinylated FABP12 antibody
The concentration of Rat CHGA in the samples is then determined by comparing the OD of the samples to the standard curve
Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of Ceramide in the sample
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 40 nm
Rat GAL (Galanin peptides) ELISA Kit SDF1 Then Streptavidin-HRP is added andThe Rat GAL (Galanin peptides) ELISA Kit measures Gal in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Gal antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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