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Then a biotinylated detection antibody specific for Mouse F9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
CASP1 present in the sample is added and binds to antibodies coated on the wells
The microplate provided in this kit has been precoated with CRH
Then Streptavidin-HRP is added and binds to the Biotinylated GSC2 antibody
Human EGF (Epidermal Growth Factor) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human EGF (Epidermal Growth Factor) ELISA Kit measures EGF in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti EGF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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