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Each set of primers has been verified by amplification curve efficiency (E>98%
coli expression system and the target gene encoding Gly24-Ala155 is expressed
After centrifugation with chloroform
coli expression system and the target gene encoding Tyr29-Gln159(Glu37Gln) is expressed with a 6His tag at the C-terminus
Mouse Amyloid Beta Peptide 1-42 ELISA Kit Packaging:96 wells Each set of primers hasThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Mouse Ab1 42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Mouse Ab1 42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
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