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Calculate the concentration of BGP in the sample by plotting standard curve
Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins
Then Streptavidin-HRP is added and binds to the Biotinylated STMN2 antibody
Then a biotinylated detection antibody specific for Mouse SHH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Sheep CALPROTECTIN (S100A8/S100A9 Heterodimer) ELISA Kit Packaging:96 wells Calculate the concentration of BGPThe Sheep CALPROTECTIN (S100A8 S100A9 Heterodimer) ELISA Kit measures S100A8 S100A9 in Sheep Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Calprotectin antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components.
