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Then a biotinylated detection antibody specific for Human IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Mouse ADP/Acrp30
The OD value is proportional to the concentration of Rat CKMB
only those wells that contain Goat IFNg
Rat IL-12p40 ELISA Kit GSD11 Then a biotinylated detection antibodyThe Rat IL 12p40 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat IL 12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat IL 12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only
