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Calculate the concentration of ETFDH in the sample by plotting standard curve
The concentration of Rat VTN in the samples is then determined by comparing the OD of the samples to the standard curve
Add HRP-detection antibody
Then a biotinylated detection antibody specific for Chicken EMMPRIN/CD147 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Mouse NES1 (Nesfatin 1) ELISA Kit TNFA Calculate the concentration of ETFDHThe Mouse NES1 (Nesfatin 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse NES1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NES1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
