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Substrate solution is then added and color develops in proportion to the amount of Human LRAT
The concentration of Human SALL4 in the samples is then determined by comparing the OD of the samples to the standard curve
only those wells that contain Mouse IL4
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MDC
Human NRTN (Neurturin) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (NRTN) Neurturin ELISA Kit measures Neurturin in samples. The plate has been pre coated with Human NRTN antibody. NRTN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NRTN Antibody is added and binds to NRTN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NRTN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
