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RNASE7 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NCAM
Then a biotinylated detection antibody specific for Human NPHN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL2
Human TOP1 (DNA topoisomerase 1) ELISA Kit Packaging:96 wells RNASE7 present in the sampleThe Human TOP1 (DNA topoisomerase 1) ELISA Kit measures TOP1 in Human Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TOP1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with TOP1 bound to
