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Then a biotinylated detection antibody specific for Human COL7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then a biotinylated detection antibody specific for Human IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian IFNg
The OD value is proportional to the concentration of Human OSM
Rat Leptin ELISA Kit IL4 Then a biotinylated detection antibodyThe Rat Leptin ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells
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