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The substrate solution is added to each well
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1ra/IL-1F3
Then a biotinylated detection antibody specific for Rat IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Human PDGF-AB
QuicKey Pro Sheep Cortisol ELISA Kit PYY4 The substrate solution is addedThe QuicKey Pro Sheep Cortisol ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Sheep Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep Cortisol are added to the micro ELISA plate wells. Sheep Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection
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