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Substrate solution is then added and color develops in proportion to the amount of Human CD84
and the color intensity is directly proportional to the amount of Ferret CD4 present
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CTSZ
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT18
Human FZD5 (Frizzled-5) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (FZD5) Frizzled 5 ELISA Kit measures Frizzled 5 in samples. The plate has been pre coated with Human FZD5 antibody. FZD5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FZD5 Antibody is added and binds to FZD5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FZD5 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
