Rat UCN (Urocortin) ELISA KIT TGFA Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Human TSP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Rat GT in the sample or standard competes with a fixed amount of Rat GT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat GT

Only those wells that contain APOD

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PLA2G2D

Rat UCN (Urocortin) ELISA KIT TGFA Then a biotinylated detection antibodyThe Rat (UCN) Urocortin ELISA KIT measures Urocortin in Rat samples. The plate has been pre coated with Rat UCN antibody. UCN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat UCN Antibody is added and binds to UCN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated UCN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution