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Substrate solution is then added and color develops in proportion to the amount of Human FGF7
Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of IL-1b in the sample
Then a biotinylated detection antibody specific for Rat C1q and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Porcine CAST
Guinea pig MMP-7 (Matrix Metalloproteinase 7) ELISA Kit LD78ALPHA Substrate solution is then addedThe Guinea pig MMP 7 (Matrix Metalloproteinase 7) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Guinea pig MMP 7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig MMP 7 and Avidin Horseradish Peroxidase (HRP) conjugate are added
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