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The concentration of Goat TNFa in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human AGP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Human TPM2
GKN2 present in the sample is added and binds to antibodies coated on the wells
Human IMA (Ischemia Modified Albumin) QuickTest ELISA Kit Packaging:96 wells The concentration of Goat TNFaThe Human IMA (Ischemia Modified Albumin) QuickTest ELISA Kit measures IMA in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples
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