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FN1 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CSN1
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRKAa1
The concentration of Mouse GMCSF in the samples is then determined by comparing the OD of the samples to the standard curve
Human CCL24 (Eotaxin 2) ELISA Kit Pancreatic icosapeptide FN1 present in the sampleThe Human (CCL24) Eotaxin 2 ELISA Kit measures Eotaxin 2 in samples. The plate has been pre coated with Human CCL24 antibody. CCL24 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CCL24 Antibody is added and binds to CCL24 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CCL24 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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