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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat eIF4E
The concentration of Human FGb in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated CBS antibody
The Mouse FATP5 (Fatty Acid Transport Protein 5) ELISA Kit is a Sandwich enzyme immunoassay
Human HTN3 (Histatin-3) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (HTN3) Histatin 3 ELISA Kit measures Histatin 3 in samples. The plate has been pre coated with Human HTN3 antibody. HTN3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HTN3 Antibody is added and binds to HTN3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HTN3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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