Rat IgG2a (Immunoglobulin G2a) ELISA Kit Packaging:48 wells Knocking down intracellular PARP1 levels

Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks

ITGB1BP1 present in the sample is added and binds to antibodies coated on the wells

Then Streptavidin-HRP is added and binds to the Biotinylated TGFBR3 antibody

Then a biotinylated detection antibody specific for Human MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Rat IgG2a (Immunoglobulin G2a) ELISA Kit Packaging:48 wells Knocking down intracellular PARP1 levelsThe Rat IgG2a (Immunoglobulin G2a) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat IgG2a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgG2a and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate