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The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MFAP5
Then a biotinylated detection antibody specific for Mouse PTX3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The CLEC11A (C-Type Lectin Domain Family 11
ITGa9 (Integrin Alpha 9) ELISA Kit (Small Sample) Packaging:48 wells The optical density (OD) isThe ITGa9 (Integrin Alpha 9) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human ITGa9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human ITGa9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
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