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Substrate solution is then added and color develops in proportion to the amount of Mouse CD83
The plate has been pre-coated with Rat Smad5 antibody
And then biotinylated Rat GPC3 Antibody is added and binds to GPC3 in the sample
The plate has been pre-coated with Rat GAL2 antibody
Human IL-4 (Interleukin 4) ELISA Kit FRCP Substrate solution is then addedThe Human IL 4 (Interleukin 4) ELISA Kit measures IL 4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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