Rat CRH (Corticotropin Releasing Hormone) ELISA Kit Packaging:96 wells The concentration of Mouse VIP

The concentration of Mouse VIP in the samples is then determined by comparing the OD of the samples to the standard curve

only those wells that contain Human TRA2b

Only those wells that contain Human CA724

Then Streptavidin-HRP is added and binds to the Biotinylated SLC2A6 antibody

Rat CRH (Corticotropin Releasing Hormone) ELISA Kit Packaging:96 wells The concentration of Mouse VIPThe Rat CRH (Corticotropin Releasing Hormone) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Rat CRH. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Rat CRH. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is