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This Granulocyte Colony-stimulating Factor ELISA kit uses the Sandwich-ELISA principle
You can calculate the concentration of Mouse Klotho in the samples by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Human C3a
Then a biotinylated detection antibody specific for Human GLα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Guinea Pig IFN-gamma ELISA Kit Packaging:48 wells This Granulocyte Colony-stimulating Factor ELISAThe Guinea Pig IFN gamma ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Guinea pig IFNg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Guinea pig IFNg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
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