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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-17
Then Streptavidin-HRP is added and binds to the Biotinylated HSPB1 antibody
FABP6 present in the sample is added and binds to antibodies coated on the wells
The LCN1 (Lipocalin 1) ELISA Kit is a Sandwich enzyme immunoassay
Human FZD1 (Frizzled-1) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (FZD1) Frizzled 1 ELISA Kit measures Frizzled 1 in samples. The plate has been pre coated with Human FZD1 antibody. FZD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FZD1 Antibody is added and binds to FZD1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FZD1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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